PIM1 Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ5360
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5360-100ul100ul£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Monoclonal
Antibody Clone: ECB-16
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store the PIM1 antibody at -20°C.

Images

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IHC staining of FFPE human prostate carcinoma with PIM1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
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Western blot testing of human Jurkat cell lysate with PIM1 antibody. Predicted molecular weight ~44 kDa (PIM-1L) and ~34 kDa (PIM-1S).
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IHC staining of FFPE human prostate carcinoma with PIM1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
Western blot testing of human Jurkat cell lysate with PIM1 antibody. Predicted molecular weight ~44 kDa (PIM-1L) and ~34 kDa (PIM-1S).

Documents

Further Information

Application Details :
Western blot: 1:5000-1:10000,Immunohistochemistry (FFPE): 1:50-1:200
Application Note:
Optimal dilution of the PIM1 antibody should be determined by the researcher.
Description:
The protein encoded by the PIM1 gene belongs to the Ser/Thr protein kinase family, and PIM subfamily. This gene is expressed primarily in B-lymphoid and myeloid cell lines, and is overexpressed in hematopoietic malignancies and in prostate cancer. It plays a role in signal transduction in blood cells, contributing to both cell proliferation and survival, and thus provides a selective advantage in tumorigenesis. Both the human and orthologous mouse genes have been reported to encode two isoforms (with preferential cellular localization) resulting from the use of alternative in-frame translation initiation codons, the upstream non-AUG (CUG) and downstream AUG codons. [RefSeq]
Format :
Purified
Formulation :
Antibody in PBS with 0.02% sodium azide and 50% glycerol
Immunogen:
A synthetic peptide specific to human PIM1 was used as the immunogen for the PIM1 antibody.
Limitation:
This PIM1 antibody is available for research use only.
Localization:
Nuclear, cytoplasmic, cell membrane
Purity:
Affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
P11309