Topoisomerase II alpha Antibody / TOP2A

Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:50-1:200

Optimal dilution of the TOP2A antibody should be determined by the researcher.

The TOP2A gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. [RefSeq]

Purified

Antibody in PBS with 0.02% sodium azide and 50% glycerol

A synthetic peptide specific to human Topoisomerase II alpha / TOP2A was used as the immunogen for the TOP2A antibody.

This TOP2A antibody is available for research use only.

Nuclear

Affinity purified

Human, Mouse, Rat

P11388