Biotin-X-NHS
| Code | Size | Price |
|---|
| CDX-B0139-M025 | 25 mg | £48.00 |
Quantity:
| CDX-B0139-M100 | 100 mg | £114.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Antibody Isotype: n/a
Antibody Clone: n/a
Regulatory Status: RUO
Shipping:
Ambient
Storage:
-20°C
Documents
Further Information
Alternate Names/Synonyms:
NHS-LC-Biotin; Biotinamidohexanoic acid N-hydroxysuccinimide ester; (+)-Biotinamidocaproate N-hydroxysuccinimidyl ester; N-(+)-Biotinyl-6-aminocaproic acid N-succinimidyl ester; N-(+)-Biotinyl-6-aminohexanoic acid NHS ester; Enzotin
Appearance:
White to off-white powder.
CAS:
72040-63-2
EClass:
32160000
Form (Short):
liquid
Handling Advice:
Protect from light and moisture.
InChi:
InChI=1S/C20H30N4O6S/c25-15(7-4-3-6-14-19-13(12-31-14)22-20(29)23-19)21-11-5-1-2-8-18(28)30-24-16(26)9-10-17(24)27/h13-14,19H,1-12H2,(H,21,25)(H2,22,23,29)/t13-,14-,19-/m0/s1
InChiKey:
UVGHPGOONBRLCX-NJSLBKSFSA-N
Long Description:
Chemical. CAS: 72040-63-2. Formula: C20H30N4O6S. MW: 454.54. Biotin-X-NHS is an amino-reactive biotinylation reagent with a long spacer arm. The 6-aminocaproic acid spacer arm provides distance between the biotin and the target ligand which helps alleviate steric hindrance. Biotin-X-NHS is used to biotinylate antibodies, proteins, peptides, or amino acids by reacting with primary amines as found in the N-terminus and Lysine side chains. The Biotin-X-NHS molecule is cell membrane permeable and can be used to label intracellular proteins. Optimal reaction conditions are at a pH of 7-9. Avoid amine-containing buffers, which may compete in the acylation reaction.
MDL:
MFCD00065502
Molecular Formula:
C20H30N4O6S
Molecular Weight:
454.54
Package Type:
Vial
Product Description:
Biotin-X-NHS is an amino-reactive biotinylation reagent with a long spacer arm. The 6-aminocaproic acid spacer arm provides distance between the biotin and the target ligand which helps alleviate steric hindrance. Biotin-X-NHS is used to biotinylate antibodies, proteins, peptides, or amino acids by reacting with primary amines as found in the N-terminus and Lysine side chains. The Biotin-X-NHS molecule is cell membrane permeable and can be used to label intracellular proteins. Optimal reaction conditions are at a pH of 7-9. Avoid amine-containing buffers, which may compete in the acylation reaction.
Purity:
>97% (NMR)
SMILES:
O=C(NCCCCCC(ON1C(CCC1=O)=O)=O)CCCC[C@@H]2SC[C@@]3([H])[C@]2([H])NC(N3)=O
Solubility Chemicals:
Soluble in DMSO (20mg/ml), DMF(20mg/ml) or methanol.
Source / Host:
Synthetic.
Transportation:
Non-hazardous
UNSPSC Category:
Fluorescent Reagents
UNSPSC Number:
41105331
Use & Stability:
Stable for at least 2 years after receipt when stored at -20°C.
References
(1) G.T. Hermanson; Bioconjugate Techn. 2nd p506-545 (2008)