TMB
| Code | Size | Price |
|---|
| CDX-T0002-G001 | 1 g | £48.00 |
Quantity:
| CDX-T0002-G005 | 5 g | £102.00 |
Quantity:
| CDX-T0002-G025 | 25 g | £316.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Antibody Isotype: n/a
Antibody Clone: n/a
Regulatory Status: RUO
Shipping:
Ambient
Storage:
+4°C
Documents
Further Information
Alternate Names/Synonyms:
3,3',5,5'-Tetramethylbenzidine; BM blue; Sure Blue TMB; TMB Blotting Plus; TMB substrate; TMBZ
Appearance:
White to light yellow powder.
CAS:
54827-17-7
EClass:
32160000
Form (Short):
liquid
Handling Advice:
Protect from light and moisture.
InChi:
InChI=1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3
InChiKey:
UAIUNKRWKOVEES-UHFFFAOYSA-N
Long Description:
Chemical. CAS: 54827-17-7. Formula: C16H20N2. MW: 240.34. 3,3',5,5'-Tetramethylbenzidine (TMB) chromogenic peroxidase sensitive dye. TMB is a noncarcinogenic substitute (Ames test negative) for benzidine suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650nm. The TMB reaction may be stopped with 2M H2SO4 (resulting in a yellow color), and read at 450nm. A sensitive and specific reagent for the detection of blood, assay of haemoglobin and assay of horseradish peroxidase (HRP).
MDL:
MFCD00007748
Molecular Formula:
C16H20N2
Molecular Weight:
240.34
Package Type:
Vial
Product Description:
3,3',5,5'-Tetramethylbenzidine (TMB) chromogenic peroxidase sensitive dye. TMB is a noncarcinogenic substitute (Ames test negative) for benzidine suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650nm. The TMB reaction may be stopped with 2M H2SO4 (resulting in a yellow color), and read at 450nm. A sensitive and specific reagent for the detection of blood, assay of haemoglobin and assay of horseradish peroxidase (HRP).
Purity:
>99% (HPLC)
SMILES:
CC1=C(N)C(C)=CC(C2=CC(C)=C(N)C(C)=C2)=C1
Solubility Chemicals:
Soluble in DMSO. Insoluble in water.
Source / Host:
Synthetic.
Transportation:
Non-hazardous
UNSPSC Category:
Fluorescent Reagents
UNSPSC Number:
41105331
Use & Stability:
Stable for at least 2 years after receipt when stored at +4°C.
References
(1) R.C. Lijana & M.C. Williams; J. Lab. Clin. Med. 94, 266 (1979) | (2) E.S. Bos, et al.; J. Immunoassay 2, 187 (1981) | (3) D.B. Rye, et al.; J. Histochem. Cytochem. 32, 1145 (1984) | (4) A.K. Goka & M.J. Farthing; J. Immunoassay 8, 29 (1987) | (5) J.L. McKimm-Breschkin; J. Immunol. Methods 135, 277 (1990) | (6) A. Frey, et al.; J. Immunol. Methods 233, 47 (2000) | (7) R. Georgescu; Methods Mol. Biol. 230, 171 (2003) | (8) L. Zhang & J. Du; Spectrochim. Acta A Mol. Biomol. Spectrosc. 158, 24 (2016) | (9) C. Lei, et al.; Talanta 175, 457 (2017)