TDRD3 Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ5455
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5455-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the TDRD3 antibody at -20oC.

Images

1 / 6
Immunofluorescent staining of FFPE human intestinal cancer with TDRD3 antibody (red) at 1ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 6
IHC staining of FFPE human colon cancer with TDRD3 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
3 / 6
IHC staining of FFPE mouse small intestine with TDRD3 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
4 / 6
IHC staining of FFPE rat small intestine with TDRD3 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
5 / 6
Western blot testing of human 1) placenta, 2) HL-60, 3) HeLa, 4) PC-3, 5) HEK293, 6) A549 and 7) A431 lysate with TDRD3 antibody at 0.5ug/ml. Expected molecular weight: 73-83 kDa.
6 / 6
Western blot testing of 1) rat brain, 2) rat liver and mouse 3) brain, 4) lung, 5) liver and 6) NIH3T3 lysate with TDRD3 antibody at 0.5ug/ml. Expected molecular weight: 73-83 kDa.
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Immunofluorescent staining of FFPE human intestinal cancer with TDRD3 antibody (red) at 1ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human colon cancer with TDRD3 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE mouse small intestine with TDRD3 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE rat small intestine with TDRD3 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Western blot testing of human 1) placenta, 2) HL-60, 3) HeLa, 4) PC-3, 5) HEK293, 6) A549 and 7) A431 lysate with TDRD3 antibody at 0.5ug/ml. Expected molecular weight: 73-83 kDa.
Western blot testing of 1) rat brain, 2) rat liver and mouse 3) brain, 4) lung, 5) liver and 6) NIH3T3 lysate with TDRD3 antibody at 0.5ug/ml. Expected molecular weight: 73-83 kDa.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml, Immunohistochemistry (FFPE): 1-2ug/ml, Immunofluorescence: 1-2ug/ml
Application Note:
Optimal dilution of the TDRD3 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Tudor domain-containing protein 3 is a protein that in humans is encoded by the TDRD3 gene. This gene is mapped to 13q21.2. It contains a Tudor domain and UBA protein domain and has three distinct protein isoforms. TDRD3 is a multifunctional protein that acts as a transcriptional coactivator in the nucleus and as a scaffolding protein in cytoplasmic stress granules.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids KPTNDNDEFEKQRTAAIAEVAKSKETK from the human protein were used as the immunogen for the TDRD3 antibody.
Limitation:
This TDRD3 antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q9H7E2