TH Antibody

ProSci
Product Code: PSI-13-009
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-13-009-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using TH antibody (13-009) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.
2 / 3
Immunohistochemistry of paraffin-embedded rat brain using TH Antibody (13-009) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse spinal cord using TH Antibody (13-009) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using TH antibody (13-009) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.
Immunohistochemistry of paraffin-embedded rat brain using TH Antibody (13-009) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spinal cord using TH Antibody (13-009) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
TH, tyrosine hydroxylase, HGNC:11782, TYH, DYT14, DYT5b, dystonia 14, tyrosine 3-monooxygenase
Application Note:
WB: 1:500 - 1:1000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100
Background:
The protein encoded by this gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human TH (NP_954986.2).
NCBI Gene ID #:
7054
NCBI Official Name:
tyrosine hydroxylase
NCBI Official Symbol:
TH
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 59kDa
Purification:
Affinity purification
Research Area:
Cancer, Neuroscience, Signal Transduction
Swissprot #:
P07101
User NOte:
Optimal dilutions for each application to be determined by the researcher.