FoxO3a Antibody

ProSci
Product Code: PSI-13-033
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-13-033-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 4
Western blot analysis of extracts of various cell lines, using FoxO3a antibody (13-033) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
2 / 4
Immunohistochemistry of paraffin-embedded rat brain using FoxO3a antibody (13-033) at dilution of 1:100 (40x lens).
3 / 4
Immunohistochemistry of paraffin-embedded human liver damage using FoxO3a antibody (13-033) at dilution of 1:100 (40x lens).
4 / 4
Immunofluorescence analysis of U2OS cells using FoxO3a antibody (13-033) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using FoxO3a antibody (13-033) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
Immunohistochemistry of paraffin-embedded rat brain using FoxO3a antibody (13-033) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver damage using FoxO3a antibody (13-033) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using FoxO3a antibody (13-033) at dilution of 1:100. Blue: DAPI for nuclear staining.

Further Information

Additional Names:
FOXO3 Antibody: FOXO2, AF6q21, FKHRL1, FOXO3A, FKHRL1P2, Forkhead box protein O3, AF6q21 protein
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 394-672 of mouse FoxO3a (NP_062714.1).
NCBI Gene ID #:
56484
NCBI Official Name:
forkhead box O3
NCBI Official Symbol:
FOXO3
NCBI Organism:
Mus musculus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 90kDa
Purification:
Affinity purification
Research Area:
Autophagy, Cell Cycle, Immunology, Signal Transduction, Transcription
Swissprot #:
O43524
User NOte:
Optimal dilutions for each application to be determined by the researcher.