GRIA2 Antibody

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ProSci
Product Code: PSI-13-036
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-13-036-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using GRIA2 antibody (13-036) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
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Immunofluorescence analysis of rat brain using GRIA2 antibody (13-036) at dilution of 1:50. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of mouse brain using GRIA2 antibody (13-036) at dilution of 1:50. Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using GRIA2 antibody (13-036) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunofluorescence analysis of rat brain using GRIA2 antibody (13-036) at dilution of 1:50. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of mouse brain using GRIA2 antibody (13-036) at dilution of 1:50. Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
GRIA2, GLUR2, GLURB, GluA2, HBGR2, GluR-K2
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200
Background:
Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to a family of glutamate receptors that are sensitive to alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA), and function as ligand-activated cation channels. These channels are assembled from 4 related subunits, GRIA1-4. The subunit encoded by this gene (GRIA2) is subject to RNA editing (CAG->CGG; Q->R) within the second transmembrane domain, which is thought to render the channel impermeable to Ca(2+). Human and animal studies suggest that pre-mRNA editing is essential for brain function, and defective GRIA2 RNA editing at the Q/R site may be relevant to amyotrophic lateral sclerosis (ALS) etiology. Alternative splicing, resulting in transcript variants encoding different isoforms, (including the flip and flop isoforms that vary in their signal transduction properties), has been noted for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 25-230 of human GRIA2 (NP_001077088.1).
NCBI Gene ID #:
2891
NCBI Official Name:
glutamate receptor, ionotropic, AMPA 2
NCBI Official Symbol:
GRIA2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 110kDa
Purification:
Affinity purification
Research Area:
Neuroscience
Swissprot #:
P42262
User NOte:
Optimal dilutions for each application to be determined by the researcher.