KRT17 Antibody

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ProSci
Product Code: PSI-13-043
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-13-043-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using KRT17 antibody (13-043) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
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Immunohistochemistry of paraffin-embedded rat kidney using KRT17 antibody (13-043) at dilution of 1:200 (40x lens).
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Immunofluorescence analysis of HeLa cells using KRT17 antibody (13-043) at dilution of 1:200. Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using KRT17 antibody (13-043) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded rat kidney using KRT17 antibody (13-043) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of HeLa cells using KRT17 antibody (13-043) at dilution of 1:200. Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
KRT17, K17, PC, PC2, PCHC1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes the type I intermediate filament chain keratin 17, expressed in nail bed, hair follicle, sebaceous glands, and other epidermal appendages. Mutations in this gene lead to Jackson-Lawler type pachyonychia congenita and steatocystoma multiplex.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 133-432 of human KRT17 (NP_000413.1).
NCBI Gene ID #:
3872
NCBI Official Name:
keratin 17
NCBI Official Symbol:
KRT17
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 48kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Signal Transduction
Swissprot #:
Q04695
User NOte:
Optimal dilutions for each application to be determined by the researcher.