MAPK3 Antibody

ProSci
Product Code: PSI-13-089
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-13-089-50uL50uL£440.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using MAPK3 antibody (13-089) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
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Immunohistochemistry of paraffin-embedded rat lung using MAPK3 Antibody (13-089) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human kidney using MAPK3 Antibody (13-089) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse heart using MAPK3 Antibody (13-089) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using MAPK3 antibody (13-089) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded rat lung using MAPK3 Antibody (13-089) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney using MAPK3 Antibody (13-089) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using MAPK3 Antibody (13-089) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
MAPK3, mitogen-activated protein kinase 3, HGNC:6877, ERK1, P44ERK1, P44MAPK, PRKM3, protein kinase, mitogen-activated 3 (MAP kinase 3 p44)
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MAPK3 (NP_002737.2).
NCBI Gene ID #:
5595
NCBI Official Name:
mitogen-activated protein kinase 3
NCBI Official Symbol:
MAPK3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 40kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cell Cycle, Immunology, Neuroscience, Signal Transduction, Stem Cell
Swissprot #:
P27361
User NOte:
Optimal dilutions for each application to be determined by the researcher.