FGF2 Antibody

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ProSci
Product Code: PSI-13-095
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-13-095-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of rat ovary, using FGF2 antibody (13-095) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
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Immunohistochemistry of paraffin-embedded rat lung using FGF2 antibody (13-095) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human prostate using FGF2 antibody (13-095) at dilution of 1:100 (20x lens).
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Immunohistochemistry of paraffin-embedded mouse heart using FGF2 antibody (13-095) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of C6 cells using FGF2 Polyclonal Antibody (13-095) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of NIH-3T3 cells using FGF2 Polyclonal Antibody (13-095) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of rat ovary, using FGF2 antibody (13-095) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat lung using FGF2 antibody (13-095) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate using FGF2 antibody (13-095) at dilution of 1:100 (20x lens).
Immunohistochemistry of paraffin-embedded mouse heart using FGF2 antibody (13-095) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using FGF2 Polyclonal Antibody (13-095) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using FGF2 Polyclonal Antibody (13-095) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
FGF2, Basic fibroblast growth factor, FGFB, Fibroblast growth factor 2, FGF-2, Prostatropin, BFGF, HBGF-2
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 143-288 of human FGF2 (NP_001997.5).
NCBI Gene ID #:
2247
NCBI Official Name:
fibroblast growth factor 2 (basic)
NCBI Official Symbol:
FGF2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 20kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Growth Factors, Neuroscience, Signal Transduction, Stem Cell
Swissprot #:
P09038
User NOte:
Optimal dilutions for each application to be determined by the researcher.