c-Fos Antibody

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ProSci
Product Code: PSI-13-096
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-13-096-50uL50uL£433.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using c-Fos antibody (13-096) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
2 / 4
Western blot analysis of extracts of HepG2 cells, using c-Fos antibody (13-096) at 1:1000 dilution.HepG2 cells were treated by serum-starvation overnight.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% BSA.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 5min.
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Immunohistochemistry of paraffin-embedded human lung cancer using C-FOS antibody (13-096) at dilution of 1:150 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse heart using C-FOS antibody (13-096) at dilution of 1:150 (40x lens).
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Western blot analysis of extracts of various cell lines, using c-Fos antibody (13-096) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Western blot analysis of extracts of HepG2 cells, using c-Fos antibody (13-096) at 1:1000 dilution.HepG2 cells were treated by serum-starvation overnight.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% BSA.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 5min.
Immunohistochemistry of paraffin-embedded human lung cancer using C-FOS antibody (13-096) at dilution of 1:150 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using C-FOS antibody (13-096) at dilution of 1:150 (40x lens).

Documents

Further Information

Additional Names:
FOS, p55, AP-1, C-FOS
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 211-380 of human c-Fos (NP_005243.1).
NCBI Gene ID #:
2353
NCBI Official Name:
FBJ murine osteosarcoma viral oncogene homolog
NCBI Official Symbol:
FOS
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 70kDa/62kDa
Purification:
Affinity purification
Research Area:
Cancer, Immunology, Neuroscience, Signal Transduction, Transcription
Swissprot #:
P01100
User NOte:
Optimal dilutions for each application to be determined by the researcher.