GFAP Antibody

ProSci
Product Code: PSI-13-097
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-13-097-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using GFAP antibody (13-097) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 2
Immunofluorescence analysis of U-251MG cells using GFAP antibody (13-097) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using GFAP antibody (13-097) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunofluorescence analysis of U-251MG cells using GFAP antibody (13-097) at dilution of 1:100. Blue: DAPI for nuclear staining.

Further Information

Additional Names:
Glial fibrillary acidic protein, GFAP, GFAP
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200
Background:
This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human GFAP (NP_002046.1).
NCBI Gene ID #:
2670
NCBI Official Name:
glial fibrillary acidic protein
NCBI Official Symbol:
GFAP
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 49kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Neuroscience, Signal Transduction, Stem Cell
Swissprot #:
P14136
User NOte:
Optimal dilutions for each application to be determined by the researcher.