HDAC1 Antibody, KO Validated

ProSci
Product Code: PSI-13-098
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-13-098-50uL50uL£505.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using HDAC1 antibody (13-098) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
2 / 6
Western blot analysis of extracts from normal (control) and HDAC1 knockout (KO) 293T cells, using HDAC1 antibody (13-098) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
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Immunohistochemistry of paraffin-embedded human prostate cancer using HDAC1 antibody (13-098) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human stomach using HDAC1 antibody (13-098) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of A549 cells using HDAC1 antibody (13-098). Blue: DAPI for nuclear staining.
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Immunoprecipitation analysis of 200ug extracts of HT-29 cells, using 3 ug HDAC1 antibody (13-098). Western blot was performed from the immunoprecipitate using HDAC1 antibody (13-098) at a dilition of 1:1000.

Western blot analysis of extracts of various cell lines, using HDAC1 antibody (13-098) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
Western blot analysis of extracts from normal (control) and HDAC1 knockout (KO) 293T cells, using HDAC1 antibody (13-098) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
Immunohistochemistry of paraffin-embedded human prostate cancer using HDAC1 antibody (13-098) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using HDAC1 antibody (13-098) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using HDAC1 antibody (13-098). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of HT-29 cells, using 3 ug HDAC1 antibody (13-098). Western blot was performed from the immunoprecipitate using HDAC1 antibody (13-098) at a dilition of 1:1000.

Further Information

Additional Names:
HDAC1 Antibody: HD1, RPD3, GON-10, RPD3L1, Histone deacetylase 1, HD1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:100 - 1:200

IF: 1:50 - 1:100

IP: 1:50 - 1:100
Background:
Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 393-482 of human HDAC1 (NP_004955.2).
NCBI Gene ID #:
3065
NCBI Official Name:
histone deacetylase 1
NCBI Official Symbol:
HDAC1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 62kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cell Cycle, Immunology, Neuroscience, Signal Transduction, Stem Cell
Swissprot #:
Q13547
User NOte:
Optimal dilutions for each application to be determined by the researcher.
VALIDATION:
Antibody is Knockout validated.