HSPB1 Antibody

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ProSci
Product Code: PSI-13-100
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-13-100-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using HSPB1 antibody (13-100) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunofluorescence analysis of C6 cells using HSPB1 antibody (13-100) at dilution of 1:100. Blue: DAPI for nuclear staining.
3 / 4
Immunofluorescence analysis of HeLa cells using HSPB1 antibody (13-100) at dilution of 1:100. Blue: DAPI for nuclear staining.
4 / 4
Immunoprecipitation analysis of 200ug extracts of HepG2 cells, using 3 ug HSPB1 antibody (13-100). Western blot was performed from the immunoprecipitate using HSPB1 antibody (13-100) at a dilition of 1:1000.
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Western blot analysis of extracts of various cell lines, using HSPB1 antibody (13-100) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunofluorescence analysis of C6 cells using HSPB1 antibody (13-100) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using HSPB1 antibody (13-100) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of HepG2 cells, using 3 ug HSPB1 antibody (13-100). Western blot was performed from the immunoprecipitate using HSPB1 antibody (13-100) at a dilition of 1:1000.

Documents

Further Information

Additional Names:
HSPB1, CMT2F, DKFZp586P1322, HS.76067, HSP27, HSP28, Hsp25, HMN2B, SRP27, HEL-S-102
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200

IP: 1:50 - 1:100
Background:
The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN).
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human HSPB1 (NP_001531.1).
NCBI Gene ID #:
3315
NCBI Official Name:
heat shock 27kDa protein 1
NCBI Official Symbol:
HSPB1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 25kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cancer, Cell Cycle, Signal Transduction, Stem Cell
Swissprot #:
P04792
User NOte:
Optimal dilutions for each application to be determined by the researcher.