GRP78 / BiP Antibody

Contact us
ProSci
Product Code: PSI-13-101
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-13-101-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 7
Western blot analysis of extracts of various cell lines, using HSPA5 antibody (13-101) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
2 / 7
Immunohistochemistry of paraffin-embedded rat brain using GRP78 / BiP antibody (13-101) at dilution of 1:100 (40x lens).
3 / 7
Immunohistochemistry of paraffin-embedded human lung cancer using GRP78 / BiP antibody (13-101) at dilution of 1:100 (40x lens).
4 / 7
Immunohistochemistry of paraffin-embedded human breast cancer using GRP78 / BiP antibody (13-101) at dilution of 1:100 (40x lens).
5 / 7
Immunohistochemistry of paraffin-embedded mouse brain using GRP78 / BiP antibody (13-101) at dilution of 1:100 (40x lens).
6 / 7
Immunofluorescence analysis of HeLa cells using GRP78 / BiP antibody (13-101) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
7 / 7
Confocal immunofluorescence analysis of U-2OS cells using GRP78 / BiP antibody (13-101) at dilution of 1:200. Blue: DAPI for nuclear staining.
❮❯

Western blot analysis of extracts of various cell lines, using HSPA5 antibody (13-101) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
Immunohistochemistry of paraffin-embedded rat brain using GRP78 / BiP antibody (13-101) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using GRP78 / BiP antibody (13-101) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using GRP78 / BiP antibody (13-101) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using GRP78 / BiP antibody (13-101) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using GRP78 / BiP antibody (13-101) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Confocal immunofluorescence analysis of U-2OS cells using GRP78 / BiP antibody (13-101) at dilution of 1:200. Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
HSPA5, BIP, Heat shock 70 kDa protein 5, MIF2, GRP-78, GRP78
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
The protein encoded by this gene is a member of the heat shock protein 70 (HSP70) family. It is localized in the lumen of the endoplasmic reticulum (ER), and is involved in the folding and assembly of proteins in the ER. As this protein interacts with many ER proteins, it may play a key role in monitoring protein transport through the cell.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 550-650 of human GRP78 / BiP (NP_005338.1).
NCBI Gene ID #:
3309
NCBI Official Name:
heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
NCBI Official Symbol:
HSPA5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 78kDa
Purification:
Affinity purification
Research Area:
Neuroscience
Swissprot #:
P11021
User NOte:
Optimal dilutions for each application to be determined by the researcher.