IKBKE Antibody

ProSci
Product Code: PSI-13-103
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-13-103-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using IKBKE antibody (13-103).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
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Immunohistochemistry of paraffin-embedded human colon carcinoma using IKBKE antibody (13-103) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded rat spleen using IKBKE antibody (13-103) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse spleen using IKBKE antibody (13-103) at dilution of 1:200 (40x lens).
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Immunofluorescence analysis of NIH/3T3 cells using IKBKE antibody (13-103) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using IKBKE antibody (13-103).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human colon carcinoma using IKBKE antibody (13-103) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat spleen using IKBKE antibody (13-103) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using IKBKE antibody (13-103) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of NIH/3T3 cells using IKBKE antibody (13-103) at dilution of 1:100. Blue: DAPI for nuclear staining.

Further Information

Additional Names:
IKK epsilon Antibody: IKKE, IKKI, IKK-E, IKK-i, IKKE, KIAA0151, Inhibitor of nuclear factor kappa-B kinase subunit epsilon, Inducible I kappa-B kinase, I-kappa-B kinase epsilon
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200

IP: 1:50 - 1:200
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 495-716 of human IKBKE (NP_054721.1).
NCBI Gene ID #:
9641
NCBI Official Name:
inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon
NCBI Official Symbol:
IKBKE
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 93kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cancer, Cell Cycle, Immunology, Innate Immunity, Signal Transduction
Swissprot #:
Q14164
User NOte:
Optimal dilutions for each application to be determined by the researcher.