KRT19 Antibody

ProSci
Product Code: PSI-13-104
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-13-104-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of mouse stomach, using KRT19 antibody (13-104) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 120s.
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Immunohistochemistry of paraffin-embedded rat kidney using KRT19 antibody (13-104) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human liver cancer using KRT19 antibody (13-104) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse kidney using KRT19 antibody (13-104) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of mouse stomach, using KRT19 antibody (13-104) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 120s.
Immunohistochemistry of paraffin-embedded rat kidney using KRT19 antibody (13-104) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using KRT19 antibody (13-104) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using KRT19 antibody (13-104) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
KRT19, CK19, K19, K1CS, MGC15366
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 241-400 of human KRT19 (NP_002267.2).
NCBI Gene ID #:
3880
NCBI Official Name:
keratin 19
NCBI Official Symbol:
KRT19
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 46kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Signal Transduction, Stem Cell
Swissprot #:
P08727
User NOte:
Optimal dilutions for each application to be determined by the researcher.