Lamin A/C Antibody

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ProSci
Product Code: PSI-13-106
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-13-106-50uL50uL£440.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using Lamin A/C antibody (13-106) at 1:1000 dilution. HeLa cells were treated by Hydroxyurea (4 mM) at 37°C for 20 hours or treated by Paclitaxel (100 nM/ml) at 37°C for 20 hours. C6 cells were treated by Hydroxyurea (4 mM) at 37°C for 20 hours or treated by Paclitaxel (100 nM) at 37°C for 20 hours. <br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
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Immunohistochemistry of paraffin-embedded Human gastric cancer using Lamin A/C antibody (13-106) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of HeLa cells using Lamin A/C antibody (13-106). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using Lamin A/C antibody (13-106) at 1:1000 dilution. HeLa cells were treated by Hydroxyurea (4 mM) at 37°C for 20 hours or treated by Paclitaxel (100 nM/ml) at 37°C for 20 hours. C6 cells were treated by Hydroxyurea (4 mM) at 37°C for 20 hours or treated by Paclitaxel (100 nM) at 37°C for 20 hours. <br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
Immunohistochemistry of paraffin-embedded Human gastric cancer using Lamin A/C antibody (13-106) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using Lamin A/C antibody (13-106). Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
LMNA, 70 kDa lamin, CDCD1, CMD1A, EMD2, FPLD, IDC, FPL, FPLD2, Lamin A/C, Lamin, LFP, LMNC, LMN1, LMNL1, HGPS, Prelamin-A/C, PRO1, Lamin A, Lamin A/C-like 1, LGMD1B, CDDC, CMT2B1, LDP1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200

IP: 1:50 - 1:200
Background:
The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Alternative splicing results in multiple transcript variants. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 403-572 of human Lamin C (NP_005563.1).
NCBI Gene ID #:
4000
NCBI Official Name:
lamin A/C
NCBI Official Symbol:
LMNA
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 69kDa, 78kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cell Cycle, Signal Transduction, Stem Cell
Swissprot #:
P02545
User NOte:
Optimal dilutions for each application to be determined by the researcher.