NME1 Antibody

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ProSci
Product Code: PSI-13-111
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-13-111-50uL50uL£433.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using NME1 antibody (13-111).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
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Immunohistochemistry of paraffin-embedded rat heart using NME1 Antibody (13-111) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using NME1 Antibody (13-111) at dilution of 1:200 (40x lens).
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Immunofluorescence analysis of A549 cells using NME1 antibody (13-111). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using NME1 antibody (13-111).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded rat heart using NME1 Antibody (13-111) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using NME1 Antibody (13-111) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of A549 cells using NME1 antibody (13-111). Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
NME1, AWD, GAAD, NB, NBS, NDPK-A, NDPKA, NM23, NM23-H1, non-metastatic cells 1, protein (NM23A) expressed in, NDP kinase A, OTTHUMP00000174772
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene (NME1) was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 90 to the C-terminus of human NME1 (NP_000260.1).
NCBI Gene ID #:
4830
NCBI Official Name:
NME/NM23 nucleoside diphosphate kinase 1
NCBI Official Symbol:
NME1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 21kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cancer, Cell Cycle, Neuroscience
Swissprot #:
P15531
User NOte:
Optimal dilutions for each application to be determined by the researcher.