VCAM1 Antibody

ProSci
Product Code: PSI-13-124
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-13-124-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using VCAM1 Antibody (13-124) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.

Western blot analysis of extracts of various cell lines, using VCAM1 Antibody (13-124) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.

Further Information

Additional Names:
Vascular cell adhesion protein 1, V-CAM 1, VCAM-1, INCAM-100, CD106, VCAM1, L1CAM
Application Note:
WB: 1:1000 - 1:3000

IHC: 1:50 - 1:200

IP: 1:50 - 1:200
Background:
This gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Three alternatively spliced transcripts encoding different isoforms have been described for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 300-400 of human VCAM1 (NP_001069.1).
NCBI Gene ID #:
7412
NCBI Official Name:
vascular cell adhesion molecule 1
NCBI Official Symbol:
VCAM1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 103kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Immunology, Neuroscience, Signal Transduction, Stem Cell
Swissprot #:
P19320
User NOte:
Optimal dilutions for each application to be determined by the researcher.