HSP70 Antibody

ProSci
Product Code: PSI-13-127
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-13-127-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using HSP70 antibody (13-127) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
2 / 3
Immunofluorescence analysis of U2OS cells using HSP70 antibody (13-127). Blue: DAPI for nuclear staining.
3 / 3
Immunofluorescence analysis of NIH/3T3 cells using HSP70 antibody (13-127) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using HSP70 antibody (13-127) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence analysis of U2OS cells using HSP70 antibody (13-127). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using HSP70 antibody (13-127) at dilution of 1:100. Blue: DAPI for nuclear staining.

Further Information

Additional Names:
HSPA1A, HSP72, HSPA1, HSP70I, HSP70-1, HSP70-1A, HEL-S-103
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200

IP: 1:50 - 1:100
Background:
This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 540 to the C-terminus of human HSP70 (NP_005336.3).
NCBI Gene ID #:
3303
NCBI Official Name:
heat shock 70kDa protein 1A
NCBI Official Symbol:
HSPA1A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 72kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cell Cycle, Neuroscience
Swissprot #:
P0DMV8
User NOte:
Optimal dilutions for each application to be determined by the researcher.