RYR2 Antibody

ProSci
Product Code: PSI-13-136
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-13-136-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of mouse heart, using RYR2 antibody (13-136) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
2 / 5
Immunohistochemistry of paraffin-embedded rat brain using RYR2 Antibody (13-136) at dilution of 1:100 (40x lens).
3 / 5
Immunohistochemistry of paraffin-embedded human placenta using RYR2 Antibody (13-136) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using RYR2 Antibody (13-136) at dilution of 1:100 (40x lens).
5 / 5
Immunofluorescence analysis of U-2 OS cells using RYR2 Polyclonal Antibody (13-136) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of mouse heart, using RYR2 antibody (13-136) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat brain using RYR2 Antibody (13-136) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human placenta using RYR2 Antibody (13-136) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using RYR2 Antibody (13-136) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U-2 OS cells using RYR2 Polyclonal Antibody (13-136) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
ARVC2, ARVD2, RYR-2, RyR, VTSIP, ryanodine receptor 2, cardiac muscle ryanodine receptor-calcium release channel, cardiac-type ryanodine receptor, islet-type ryanodine receptor, kidney-type ryanodine receptor, ryanodine receptor 2 (cardiac), type 2 ryanodine receptor
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes a ryanodine receptor found in cardiac muscle sarcoplasmic reticulum. The encoded protein is one of the components of a calcium channel, composed of a tetramer of the ryanodine receptor proteins and a tetramer of FK506 binding protein 1B proteins, that supplies calcium to cardiac muscle. Mutations in this gene are associated with stress-induced polymorphic ventricular tachycardia and arrhythmogenic right ventricular dysplasia.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 4850-4950 of human RYR2 (NP_001026.2).
NCBI Gene ID #:
6262
NCBI Official Name:
ryanodine receptor 2
NCBI Official Symbol:
RYR2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 565kDa
Purification:
Affinity purification
Research Area:
Cancer, Neuroscience, Signal Transduction
Swissprot #:
Q92736
User NOte:
Optimal dilutions for each application to be determined by the researcher.