AKT2 Antibody

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ProSci
Product Code: PSI-13-146
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-13-146-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using AKT2 antibody (13-146).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
2 / 2
Immunofluorescence analysis of HeLa cells using AKT2 antibody (13-146) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using AKT2 antibody (13-146).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence analysis of HeLa cells using AKT2 antibody (13-146) at dilution of 1:100. Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
rac protein kinase beta, Murine thymoma viral (v-akt) homolog-2, RAC-BETA, PRKBB, PKBBETA, HGNC:392, v-akt murine thymoma viral oncogene homolog 2, AKT2, PKBB, HIHGHH
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200
Background:
This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. The gene was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide of human AKT2
NCBI Gene ID #:
208
NCBI Official Name:
v-akt murine thymoma viral oncogene homolog 2
NCBI Official Symbol:
AKT2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 65kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cancer, Cell Cycle, Immunology, Neuroscience, Signal Transduction
Swissprot #:
P31751
User NOte:
Optimal dilutions for each application to be determined by the researcher.