CDK4 Antibody

ProSci
Product Code: PSI-13-154
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-13-154-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using CDK4 antibody (13-154) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
2 / 7
Western blot analysis of extracts of various cell lines, using CDK4 antibody (13-154) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 3min.
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Immunohistochemistry of paraffin-embedded rat kidney using CDK4 Antibody (13-154) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human esophagus using CDK4 Antibody (13-154) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse kidney using CDK4 Antibody (13-154) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of A-549 cells using CDK4 antibody (13-154). Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of PC12 cells using CD4 antibody (13-152) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using CDK4 antibody (13-154) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Western blot analysis of extracts of various cell lines, using CDK4 antibody (13-154) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 3min.
Immunohistochemistry of paraffin-embedded rat kidney using CDK4 Antibody (13-154) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using CDK4 Antibody (13-154) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using CDK4 Antibody (13-154) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A-549 cells using CDK4 antibody (13-154). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC12 cells using CD4 antibody (13-152) at dilution of 1:100. Blue: DAPI for nuclear staining.

Further Information

Additional Names:
CDK4, CMM3, PSK-J3
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200

IP: 1:50 - 1:200
Background:
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-303 of human CDK4 (NP_000066.1).
NCBI Gene ID #:
1019
NCBI Official Name:
cyclin-dependent kinase 4
NCBI Official Symbol:
CDK4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 34kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Signal Transduction
Swissprot #:
P11802
User NOte:
Optimal dilutions for each application to be determined by the researcher.