RARA Antibody

ProSci
Product Code: PSI-13-155
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-13-155-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using RARA antibody (13-155) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
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Immunohistochemistry of paraffin-embedded rat lung using RARA antibody (13-155) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat brain using RARA antibody (13-155) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse cerebellum using RARA antibody (13-155) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using RARA antibody (13-155) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunohistochemistry of paraffin-embedded rat lung using RARA antibody (13-155) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using RARA antibody (13-155) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse cerebellum using RARA antibody (13-155) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
RARA, RAR, NR1B1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100
Background:
This gene represents a nuclear retinoic acid receptor. The encoded protein, retinoic acid receptor alpha, regulates transcription in a ligand-dependent manner. This gene has been implicated in regulation of development, differentiation, apoptosis, granulopoeisis, and transcription of clock genes. Translocations between this locus and several other loci have been associated with acute promyelocytic leukemia. Alternatively spliced transcript variants have been found for this locus.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-457 of human RARA (NP_001019980.1).
NCBI Gene ID #:
5914
NCBI Official Name:
retinoic acid receptor, alpha
NCBI Official Symbol:
RARA
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 65kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Signal Transduction, Transcription
Swissprot #:
P10276
User NOte:
Optimal dilutions for each application to be determined by the researcher.