Fatty Acid Synthase Antibody, KO Validated

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ProSci
Product Code: PSI-13-178
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-13-178-50uL50uL£505.00
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of H460 cells, using Fatty Acid Synthase antibody (13-178) at 1:200 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Western blot analysis of extracts from normal (control) and Fatty Acid Synthase knockout (KO) 293T cells, using Fatty Acid Synthase antibody (13-178) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded human colon using Fatty Acid Synthase antibody (13-178) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human gastric cancer using Fatty Acid Synthase antibody (13-178) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of A549 cells using Fatty Acid Synthase antibody (13-178). Blue: DAPI for nuclear staining.
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Immunoprecipitation analysis of 200ug extracts of H460 cells, using 3 ug Fatty Acid Synthase antibody (13-178). Western blot was performed from the immunoprecipitate using Fatty Acid Synthase antibody (13-178) at a dilition of 1:1000.
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Western blot analysis of extracts of H460 cells, using Fatty Acid Synthase antibody (13-178) at 1:200 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Western blot analysis of extracts from normal (control) and Fatty Acid Synthase knockout (KO) 293T cells, using Fatty Acid Synthase antibody (13-178) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human colon using Fatty Acid Synthase antibody (13-178) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using Fatty Acid Synthase antibody (13-178) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using Fatty Acid Synthase antibody (13-178). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of H460 cells, using 3 ug Fatty Acid Synthase antibody (13-178). Western blot was performed from the immunoprecipitate using Fatty Acid Synthase antibody (13-178) at a dilition of 1:1000.

Documents

Further Information

Additional Names:
Fatty acid synthase, [Acyl-carrier-protein] S-acetyltransferase, [Acyl-carrier-protein] S-malonyltransferase, 3-oxoacyl-[acyl-carrier-protein] synthase, 3-oxoacyl-[acyl-carrier-protein] reductase, 3-hydroxyacyl-[acyl-carrier-protein] dehydratase, Enoyl-[acyl-carrier-protein] reductase, Oleoyl-[acyl-carrier-protein] hydrolase, FASN, FAS
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:200

IP: 1:50 - 1:100
Background:
The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 2212-2511 of human Fatty Acid Synthase (NP_004095.4).
NCBI Gene ID #:
2194
NCBI Official Name:
Fatty acid synthase
NCBI Official Symbol:
FASN
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 275kDa
Purification:
Affinity purification
Research Area:
Cancer, Neuroscience, Signal Transduction
Swissprot #:
P49327
User NOte:
Optimal dilutions for each application to be determined by the researcher.
VALIDATION:
Antibody is Knockout validated.