PRKCD Antibody

ProSci
Product Code: PSI-13-180
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-13-180-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 5
Western blot analysis of extracts of various cell lines, using PKCδ antibody (13-180) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1min.
2 / 5
Immunohistochemistry of paraffin-embedded rat lung using PKCδ antibody (13-180) at dilution of 1:100 (40x lens).
3 / 5
Immunohistochemistry of paraffin-embedded human tonsil using PKCδ antibody (13-180) at dilution of 1:100 (40x lens).
4 / 5
Immunohistochemistry of paraffin-embedded mouse testis using PKCδ antibody (13-180) at dilution of 1:100 (40x lens).
5 / 5
Immunofluorescence analysis of NIH/3T3 cells using PKCδ antibody (13-180) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using PKCδ antibody (13-180) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1min.
Immunohistochemistry of paraffin-embedded rat lung using PKCδ antibody (13-180) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human tonsil using PKCδ antibody (13-180) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using PKCδ antibody (13-180) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of NIH/3T3 cells using PKCδ antibody (13-180) at dilution of 1:100. Blue: DAPI for nuclear staining.

Further Information

Additional Names:
Protein kinase C delta type, Tyrosine-protein kinase PRKCD, nPKC-delta, Protein kinase C delta type regulatory subunit, Protein kinase C delta type catalytic subunit, Sphingosine-dependent protein kinase-1, SDK1, PRKCD
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100
Background:
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. The protein encoded by this gene is one of the PKC family members. Studies both in human and mice demonstrate that this kinase is involved in B cell signaling and in the regulation of growth, apoptosis, and differentiation of a variety of cell types. Alternatively spliced transcript variants encoding the same protein have been observed.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 477-676 of human PRKCD (NP_997704.1).
NCBI Gene ID #:
5580
NCBI Official Name:
Protein kinase C delta type
NCBI Official Symbol:
PRKCD
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 78kDa/85kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cancer, Cell Cycle, Immunology, Neuroscience, Signal Transduction
Swissprot #:
Q05655
User NOte:
Optimal dilutions for each application to be determined by the researcher.