MYCN Antibody

ProSci
Product Code: PSI-13-185
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-13-185-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using MYCN antibody (13-185) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
2 / 3
Immunohistochemistry of paraffin-embedded mouse brain using MYCN antibody (13-185) (40x lens).
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Immunohistochemistry of paraffin-embedded rat brain using MYCN antibody (13-185) (40x lens).

Western blot analysis of extracts of various cell lines, using MYCN antibody (13-185) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded mouse brain using MYCN antibody (13-185) (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using MYCN antibody (13-185) (40x lens).

Further Information

Additional Names:
MYCN, NMYC, ODED, MODED, N-myc, MYCNOT, bHLHe37
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
This gene is a member of the MYC family and encodes a protein with a basic helix-loop-helix (bHLH) domain. This protein is located in the nucleus and must dimerize with another bHLH protein in order to bind DNA. Amplification of this gene is associated with a variety of tumors, most notably neuroblastomas. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 168-267 of human MYCN (NP_005369.2).
NCBI Gene ID #:
4613
NCBI Official Name:
v-myc avian myelocytomatosis viral oncogene neuroblastoma derived homolog
NCBI Official Symbol:
MYCN
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 49kDa
Purification:
Affinity purification
Research Area:
Cancer, Signal Transduction, Transcription
Swissprot #:
P04198
User NOte:
Optimal dilutions for each application to be determined by the researcher.