NUMA1 Antibody

ProSci
Product Code: PSI-13-194
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-13-194-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using NUMA1 antibody (13-194) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded human stomach using NUMA1 antibody (13-194) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat heart using NUMA1 antibody (13-194) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse stomach using NUMA1 antibody (13-194) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of U2OS cells using NUMA1 antibody (13-194) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using NUMA1 antibody (13-194) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human stomach using NUMA1 antibody (13-194) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using NUMA1 antibody (13-194) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse stomach using NUMA1 antibody (13-194) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using NUMA1 antibody (13-194) at dilution of 1:100. Blue: DAPI for nuclear staining.

Further Information

Additional Names:
NMP-22, NUMA, nuclear mitotic apparatus protein 1, SP-H antigen, centrophilin stabilizes mitotic spindle in mitotic cells, nuclear matrix protein-22, structural nuclear protein
Application Note:
WB: 1:200 - 1:1000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100
Background:
This gene encodes a large protein that forms a structural component of the nuclear matrix. The encoded protein interacts with microtubules and plays a role in the formation and organization of the mitotic spindle during cell division. Chromosomal translocation of this gene with the RARA (retinoic acid receptor, alpha) gene on chromosome 17 have been detected in patients with acute promyelocytic leukemia. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-309 of human NUMA1 (NP_006176.2).
NCBI Gene ID #:
4926
NCBI Official Name:
nuclear mitotic apparatus protein 1
NCBI Official Symbol:
NUMA1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 238kDa
Purification:
Affinity purification
Research Area:
Cell Cycle
Swissprot #:
Q14980
User NOte:
Optimal dilutions for each application to be determined by the researcher.