HSPA9 Antibody

ProSci
Product Code: PSI-13-203
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-13-203-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using HSPA9 antibody (13-203) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
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Immunohistochemistry of paraffin-embedded rat kidney using HSPA9 antibody (13-203) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human lung cancer using HSPA9 antibody (13-203) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human gastric cancer using HSPA9 antibody (13-203) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse heart using HSPA9 antibody (13-203) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of U2OS cells using HSPA9 antibody (13-203). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using HSPA9 antibody (13-203) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded rat kidney using HSPA9 antibody (13-203) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using HSPA9 antibody (13-203) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using HSPA9 antibody (13-203) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using HSPA9 antibody (13-203) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using HSPA9 antibody (13-203). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
HSPA9, Heat shock 70 kDa protein 9, GRP-75, Heat shock 70kD protein 9B, GRP75, Mortalin-2, Mortalin, perinuclear, Mot-2, MOT2, Peptide-binding protein 74, MOT, MTHSP75, p66-mortalin, PBP74, CSA, HSPA9B, Mortalin
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes a member of the heat shock protein 70 gene family. The encoded protein is primarily localized to the mitochondria but is also found in the endoplasmic reticulum, plasma membrane and cytoplasmic vesicles. This protein is a heat-shock cognate protein. This protein plays a role in cell proliferation, stress response and maintenance of the mitochondria. A pseudogene of this gene is found on chromosome 2.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 380-679 of human HSPA9 (NP_004125.3).
NCBI Gene ID #:
3313
NCBI Official Name:
heat shock 70kDa protein 9 (mortalin)
NCBI Official Symbol:
HSPA9
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 80kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cancer, Cell Cycle, Signal Transduction
Swissprot #:
P38646
User NOte:
Optimal dilutions for each application to be determined by the researcher.