NOTCH2 Antibody

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ProSci
Product Code: PSI-13-204
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-13-204-50uL50uL£433.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using NOTCH2 antibody (13-204) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
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Immunohistochemistry of paraffin-embedded human stomach using NOTCH2 antibody (13-204) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using NOTCH2 antibody (13-204) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of various cell lines, using NOTCH2 antibody (13-204) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
Immunohistochemistry of paraffin-embedded human stomach using NOTCH2 antibody (13-204) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using NOTCH2 antibody (13-204) at dilution of 1:100 (40x lens).

Documents

Further Information

Additional Names:
NOTCH2, Notch homolog 2 (Drosophila), HGNC:7882, hN2, Notch (Drosophila) homolog 2, OTTHUMP00000059536, notch 2, AGS2, HJCYS
Application Note:
WB: 1:500 - 1:2000

IHC: 1:100 - 1:200
Background:
This gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play a role in vascular, renal and hepatic development. Two transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 2242-2471 of human NOTCH2 (NP_077719.2).
NCBI Gene ID #:
4853
NCBI Official Name:
notch 2
NCBI Official Symbol:
NOTCH2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 120kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cell Cycle, Neuroscience, Signal Transduction, Stem Cell
Swissprot #:
Q04721
User NOte:
Optimal dilutions for each application to be determined by the researcher.