PEBP1 Antibody

ProSci
Product Code: PSI-13-208
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-13-208-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 3
Western blot analysis of extracts of various cell lines, using PEBP1 antibody (13-208) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
2 / 3
Immunofluorescence analysis of HeLa cells using PEBP1 antibody (13-208). Blue: DAPI for nuclear staining.
3 / 3
Immunoprecipitation analysis of 150ug extracts of MCF-7 cells using 3ug PEBP1 antibody (13-208). Western blot was performed from the immunoprecipitate using PEBP1 antibody (13-208) at a dilition of 1:500.

Western blot analysis of extracts of various cell lines, using PEBP1 antibody (13-208) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunofluorescence analysis of HeLa cells using PEBP1 antibody (13-208). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 150ug extracts of MCF-7 cells using 3ug PEBP1 antibody (13-208). Western blot was performed from the immunoprecipitate using PEBP1 antibody (13-208) at a dilition of 1:500.

Further Information

Additional Names:
PEBP1, HCNP, PBP, PEBP, RKIP, HCNPpp, PEBP-1, HEL-210, HEL-S-34
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200

IP: 1:20 - 1:50
Background:
This gene encodes a member of the phosphatidylethanolamine-binding family of proteins and has been shown to modulate multiple signaling pathways, including the MAP kinase (MAPK), NF-kappa B, and glycogen synthase kinase-3 (GSK-3) signaling pathways. The encoded protein can be further processed to form a smaller cleavage product, hippocampal cholinergic neurostimulating peptide (HCNP), which may be involved in neural development. This gene has been implicated in numerous human cancers and may act as a metastasis suppressor gene. Multiple pseudogenes of this gene have been identified in the genome.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-187 of human PEBP1 (NP_002558.1).
NCBI Gene ID #:
5037
NCBI Official Name:
phosphatidylethanolamine binding protein 1
NCBI Official Symbol:
PEBP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 22kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Neuroscience
Swissprot #:
P30086
User NOte:
Optimal dilutions for each application to be determined by the researcher.