YES1 Antibody

ProSci
Product Code: PSI-13-225
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-13-225-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 2
Western blot analysis of extracts of various cell lines, using YES1 antibody (13-225) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 20s.
2 / 2
Immunohistochemistry of paraffin-embedded mouse brain using YES1 antibody (13-225) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using YES1 antibody (13-225) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 20s.
Immunohistochemistry of paraffin-embedded mouse brain using YES1 antibody (13-225) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
YES1, C-yes, Cyes, Cellular yes-1 protein, HsT441, Oncogene kinase yes1, p61-YES, p61yes, p62c-yes, Proto-oncogene c-Yes, Tyrosine-protein kinase Yes, Yes kinase, v-yes, Yes, Yamaguchi sarcoma oncogene
Application Note:
WB: 1:500 - 1:1000

IHC: 1:50 - 1:100
Background:
This gene is the cellular homolog of the Yamaguchi sarcoma virus oncogene. The encoded protein has tyrosine kinase activity and belongs to the src family of proteins. This gene lies in close proximity to thymidylate synthase gene on chromosome 18, and a corresponding pseudogene has been found on chromosome 22.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human YES1 (NP_005424.1).
NCBI Gene ID #:
7525
NCBI Official Name:
v-yes-1 Yamaguchi sarcoma viral oncogene homolog 1
NCBI Official Symbol:
YES1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 61kDa
Purification:
Affinity purification
Research Area:
Cancer, Immunology, Signal Transduction
Swissprot #:
P07947
User NOte:
Optimal dilutions for each application to be determined by the researcher.