ATP1A Antibody

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ProSci
Product Code: PSI-13-233
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-13-233-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of LO2 cells, using ATP1A1 Antibody (13-233) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
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Immunohistochemistry of paraffin-embedded human prostate using ATP1A1 antibody (13-233) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human stomach using ATP1A1 antibody (13-233) at dilution of 1:100 (40x lens).
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Immunoprecipitation analysis of 200ug extracts of LO2 cells, using 3 ug ATP1A1 antibody (13-233). Western blot was performed from the immunoprecipitate using ATP1A1 antibody (13-233) at a dilition of 1:1000.
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Western blot analysis of extracts of LO2 cells, using ATP1A1 Antibody (13-233) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human prostate using ATP1A1 antibody (13-233) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using ATP1A1 antibody (13-233) at dilution of 1:100 (40x lens).
Immunoprecipitation analysis of 200ug extracts of LO2 cells, using 3 ug ATP1A1 antibody (13-233). Western blot was performed from the immunoprecipitate using ATP1A1 antibody (13-233) at a dilition of 1:1000.

Documents

Further Information

Additional Names:
Sodium/potassium-transporting ATPase subunit alpha-1, Na(+)/K(+) ATPase alpha-1 subunit, 3.6.3.9, Sodium pump subunit alpha-1, ATP1A1
Application Note:
WB: 1:500 - 1:1000

IHC: 1:50 - 1:200

IP: 1:50 - 1:100
Background:
The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 551-850 of human ATP1A (NP_000692.2).
NCBI Gene ID #:
476/477/478/480
NCBI Official Name:
Sodium/potassium-transporting ATPase subunit alpha-1
NCBI Official Symbol:
ATP1A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 113kDa
Purification:
Affinity purification
Research Area:
Signal Transduction
Swissprot #:
P05023
User NOte:
Optimal dilutions for each application to be determined by the researcher.