ZO-1 Antibody

ProSci
Product Code: PSI-13-238
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-13-238-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using ZO-1 antibody (13-238) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 15s.

Western blot analysis of extracts of various cell lines, using ZO-1 antibody (13-238) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 15s.

Further Information

Additional Names:
ZO-1, zonula occludens 1 protein, zona occludens 1, tight junction protein ZO-1, tight junction protein 1, OTTHUMP00000174520, ZO-1, MGC133289, DKFZp686M05161, tight junction protein 1 (zona occludens 1), TJP1, ZO1
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200
Background:
This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions. Alternative splicing of this gene results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1600-1700 of human ZO-1 (NP_003248.3).
NCBI Gene ID #:
7082
NCBI Official Name:
tight junction protein 1
NCBI Official Symbol:
TJP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 250kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q07157
User NOte:
Optimal dilutions for each application to be determined by the researcher.