GATA2 Antibody

ProSci
Product Code: PSI-13-242
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-13-242-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using GATA2 antibody (13-242) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
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Immunohistochemistry of paraffin-embedded mouse brain using GATA2 antibody (13-242) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat brain using GATA2 antibody (13-242) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using GATA2 antibody (13-242) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded mouse brain using GATA2 antibody (13-242) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using GATA2 antibody (13-242) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
GATA2, DCML, NFE1B, MONOMAC
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
This gene encodes a member of the GATA family of zinc-finger transcription factors that are named for the consensus nucleotide sequence they bind in the promoter regions of target genes. The encoded protein plays an essential role in regulating transcription of genes involved in the development and proliferation of hematopoietic and endocrine cell lineages. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human GATA2 (NP_116027.2).
NCBI Gene ID #:
2624
NCBI Official Name:
GATA binding protein 2
NCBI Official Symbol:
GATA2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 50kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Stem Cell, Transcription
Swissprot #:
P23769
User NOte:
Optimal dilutions for each application to be determined by the researcher.