FGF1 Antibody

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ProSci
Product Code: PSI-13-246
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-13-246-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of 293T cells, using FGF1 antibody (13-246) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 20s.
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Immunohistochemistry of paraffin-embedded mouse brain using FGF1 antibody (13-246) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat brain using FGF1 antibody (13-246) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of 293T cells, using FGF1 antibody (13-246) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 20s.
Immunohistochemistry of paraffin-embedded mouse brain using FGF1 antibody (13-246) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using FGF1 antibody (13-246) at dilution of 1:100 (40x lens).

Documents

Further Information

Additional Names:
FGF1, AFGF, Endothelial cell growth factor, Fibroblast growth factor 1, FGFA, ECGFB, HBGF-1, GLIO703, ECGF, ECGF-beta, ECGFA, FGF-1, FGF-alpha, HBGF1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:100 - 1:200
Background:
The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Multiple alternatively spliced variants encoding different isoforms have been described.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 16-155 of human FGF1 (NP_001138364.1).
NCBI Gene ID #:
2246
NCBI Official Name:
fibroblast growth factor 1 (acidic)
NCBI Official Symbol:
FGF1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 17kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Growth Factors, Signal Transduction
Swissprot #:
P05230
User NOte:
Optimal dilutions for each application to be determined by the researcher.