DES Antibody

ProSci
Product Code: PSI-13-255
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-13-255-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 6
Western blot analysis of extracts of mouse heart, using DES antibody (13-255).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
2 / 6
Immunohistochemistry of paraffin-embedded human appendix using DES antibody (13-255) at dilution of 1:100 (40x lens).
3 / 6
Immunohistochemistry of paraffin-embedded rat ovary using DES antibody (13-255) at dilution of 1:100 (40x lens).
4 / 6
Immunohistochemistry of paraffin-embedded mouse heart using DES antibody (13-255) at dilution of 1:100 (40x lens).
5 / 6
Immunofluorescence analysis of mouse heart using DES antibody (13-255) at dilution of 1:100. Blue: DAPI for nuclear staining.
6 / 6
Immunofluorescence analysis of rat heart using DES antibody (13-255) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of mouse heart, using DES antibody (13-255).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human appendix using DES antibody (13-255) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat ovary using DES antibody (13-255) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using DES antibody (13-255) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of mouse heart using DES antibody (13-255) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of rat heart using DES antibody (13-255) at dilution of 1:100. Blue: DAPI for nuclear staining.

Further Information

Additional Names:
DES, CMD1I, CSM1, CSM2, FLJ12025, FLJ39719, FLJ41013, FLJ41793, LGMD2R
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100
Background:
This gene encodes a muscle-specific class III intermediate filament. Homopolymers of this protein form a stable intracytoplasmic filamentous network connecting myofibrils to each other and to the plasma membrane. Mutations in this gene are associated with desmin-related myopathy, a familial cardiac and skeletal myopathy (CSM), and with distal myopathies.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 121-470 of human DES (NP_001918.3).
NCBI Gene ID #:
1674
NCBI Official Name:
desmin
NCBI Official Symbol:
DES
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 54kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Signal Transduction, Stem Cell
Swissprot #:
P17661
User NOte:
Optimal dilutions for each application to be determined by the researcher.