EZR Antibody

Contact us
ProSci
Product Code: PSI-13-256
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-13-256-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 5
Western blot analysis of extracts of various cell lines, using EZR antibody (13-256) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
2 / 5
Immunohistochemistry of paraffin-embedded human stomach using EZR antibody (13-256) at dilution of 1:100 (40x lens).
3 / 5
Immunofluorescence analysis of C6 cells using EZR antibody (13-256) at dilution of 1:100. Blue: DAPI for nuclear staining.
4 / 5
Immunofluorescence analysis of U-2 OS cells using EZR antibody (13-256) at dilution of 1:100. Blue: DAPI for nuclear staining.
5 / 5
Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug EZR antibody (13-256). Western blot was performed from the immunoprecipitate using EZR antibody (13-256) at a dilition of 1:1000.
❮❯

Western blot analysis of extracts of various cell lines, using EZR antibody (13-256) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human stomach using EZR antibody (13-256) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using EZR antibody (13-256) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using EZR antibody (13-256) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug EZR antibody (13-256). Western blot was performed from the immunoprecipitate using EZR antibody (13-256) at a dilition of 1:1000.

Documents

Further Information

Additional Names:
Ezrin, VIL2, villin 2 (ezrin), CVIL, CVL, DKFZp762H157, FLJ26216, MGC1584, cytovillin, ezrin, villin 2
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100

IP: 1:50 - 1:100
Background:
The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 350-470 of human EZR (NP_003370.2).
NCBI Gene ID #:
7430
NCBI Official Name:
ezrin
NCBI Official Symbol:
EZR
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 90kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Immunology, Signal Transduction
Swissprot #:
P15311
User NOte:
Optimal dilutions for each application to be determined by the researcher.