ALK Antibody

Contact us
ProSci
Product Code: PSI-13-278
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-13-278-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at 4°C. Avoid freeze / thaw cycles.

Images

1 / 1
Western blot analysis of extracts of A-431 cells, using ALK antibody (13-278) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
❮❯

Western blot analysis of extracts of A-431 cells, using ALK antibody (13-278) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.

Documents

Further Information

Additional Names:
CD246, NBLST3
Application Note:
WB: 1:500 - 1:1000

IHC: 1:50 - 1:100
Background:
This gene encodes a receptor tyrosine kinase, which belongs to the insulin receptor superfamily. This protein comprises an extracellular domain, an hydrophobic stretch corresponding to a single pass transmembrane region, and an intracellular kinase domain. It plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. This gene has been found to be rearranged, mutated, or amplified in a series of tumours including anaplastic large cell lymphomas, neuroblastoma, and non-small cell lung cancer. The chromosomal rearrangements are the most common genetic alterations in this gene, which result in creation of multiple fusion genes in tumourigenesis, including ALK (chromosome 2)/EML4 (chromosome 2), ALK/RANBP2 (chromosome 2), ALK/ATIC (chromosome 2), ALK/TFG (chromosome 3), ALK/NPM1 (chromosome 5), ALK/SQSTM1 (chromosome 5), ALK/KIF5B (chromosome 10), ALK/CLTC (chromosome 17), ALK/TPM4 (chromosome 19), and ALK/MSN (chromosome X).
Buffer:
PBS with 0.02% sodium azide, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 19-300 of human ALK (NP_004295.2).
NCBI Gene ID #:
238
NCBI Official Name:
anaplastic lymphoma receptor tyrosine kinase
NCBI Official Symbol:
ALK
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 185kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Growth Factors, Immunology, Neuroscience, Signal Transduction
Swissprot #:
Q9UM73
User NOte:
Optimal dilutions for each application to be determined by the researcher.