CALB1 Antibody

ProSci
Product Code: PSI-13-283
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-13-283-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using CALB1 antibody (13-283) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
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Immunohistochemistry of paraffin-embedded rat kidney using CALB1 antibody (13-283) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse kidney using CALB1 antibody (13-283) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using CALB1 antibody (13-283) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat kidney using CALB1 antibody (13-283) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using CALB1 antibody (13-283) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
CALB1, calbindin 1, 28kDa, CALB, D-28K, OTTHUMP00000166027, OTTHUMP00000225441, RTVL-H protein, calbindin, calbindin 1, (28kD), calbindin D28, vitamin D-dependent calcium-binding protein, avian-type
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100
Background:
The protein encoded by this gene is a member of the calcium-binding protein superfamily that includes calmodulin and troponin C. Originally described as a 27 kDa protein, it is now known to be a 28 kDa protein. It contains four active calcium-binding domains, and has two modified domains that are thought to have lost their calcium binding capability. This protein is thought to buffer entry of calcium upon stimulation of glutamate receptors. Depletion of this protein was noted in patients with Huntington disease.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-261 of human CALB1 (NP_004920.1).
NCBI Gene ID #:
793
NCBI Official Name:
calbindin 1, 28kDa
NCBI Official Symbol:
CALB1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 30kDa
Purification:
Affinity purification
Research Area:
Neuroscience
Swissprot #:
P05937
User NOte:
Optimal dilutions for each application to be determined by the researcher.