PTCH1 Antibody

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ProSci
Product Code: PSI-13-291
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-13-291-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using PTCH1 antibody (13-291) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunofluorescence analysis of C6 cells using PTCH1 antibody (13-291) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of C6 cells using PTCH1 antibody (13-291) at dilution of 1:100. Blue: DAPI for nuclear staining.
4 / 6
Immunofluorescence analysis of mouse embryos cells using PTCH1 antibody (13-291) at dilution of 1:100. Blue: DAPI for nuclear staining.
5 / 6
Immunofluorescence analysis of C6 cells using PTCH1 antibody (13-291) at dilution of 1:100. Blue: DAPI for nuclear staining.
6 / 6
Immunofluorescence analysis of mouse embryos cells using PTCH1 antibody (13-291) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using PTCH1 antibody (13-291) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunofluorescence analysis of C6 cells using PTCH1 antibody (13-291) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of C6 cells using PTCH1 antibody (13-291) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of mouse embryos cells using PTCH1 antibody (13-291) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of C6 cells using PTCH1 antibody (13-291) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of mouse embryos cells using PTCH1 antibody (13-291) at dilution of 1:100. Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
PTCH1, PTC, BCNS, HPE7, PTC1, PTCH, NBCCS, PTCH11
Application Note:
WB: 1:500 - 1:1000

IF: 1:50 - 1:200
Background:
This gene encodes a member of the patched gene family. The encoded protein is the receptor for sonic hedgehog, a secreted molecule implicated in the formation of embryonic structures and in tumorigenesis, as well as the desert hedgehog and indian hedgehog proteins. This gene functions as a tumor suppressor. Mutations of this gene have been associated with basal cell nevus syndrome, esophageal squamous cell carcinoma, trichoepitheliomas, transitional cell carcinomas of the bladder, as well as holoprosencephaly. Alternative splicing results in multiple transcript variants encoding different isoforms. Additional splice variants have been described, but their full length sequences and biological validity cannot be determined currently.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1214-1312 of human PTCH1 (NP_000255.2).
NCBI Gene ID #:
5727
NCBI Official Name:
patched 1
NCBI Official Symbol:
PTCH1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 160kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Neuroscience, Signal Transduction, Stem Cell
Swissprot #:
Q13635
User NOte:
Optimal dilutions for each application to be determined by the researcher.