ILK Antibody

ProSci
Product Code: PSI-13-317
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-13-317-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using ILK antibody (13-317) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
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Immunohistochemistry of paraffin-embedded rat kidney using ILK Antibody (13-317) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using ILK Antibody (13-317) at dilution of 1:200 (40x lens).

Western blot analysis of extracts of various cell lines, using ILK antibody (13-317) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded rat kidney using ILK Antibody (13-317) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using ILK Antibody (13-317) at dilution of 1:200 (40x lens).

Further Information

Additional Names:
p59ILK, integrin-linked protein kinase, integrin-linked kinase-2, ILK-1, 59 kDa serine/threonine-protein kinase, ILK-2, DKFZp686F1765, P59, integrin-linked kinase, ILK, ILK1, ILK2
Application Note:
WB: 1:500 - 1:1000

IHC: 1:50 - 1:100
Background:
This gene encodes a protein with a kinase-like domain and four ankyrin-like repeats. The encoded protein associates at the cell membrane with the cytoplasmic domain of beta integrins, where it regulates integrin-mediated signal transduction. Activity of this protein is important in the epithelial to mesenchymal transition, and over-expression of this gene is implicated in tumor growth and metastasis. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human ILK (NP_001014795.1).
NCBI Gene ID #:
3611
NCBI Official Name:
integrin-linked kinase
NCBI Official Symbol:
ILK
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 48kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Signal Transduction
Swissprot #:
Q13418
User NOte:
Optimal dilutions for each application to be determined by the researcher.