CEBPA Antibody

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ProSci
Product Code: PSI-13-318
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-13-318-50uL50uL£433.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of mouse lung, using CEBPA antibody (13-318) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
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Immunohistochemistry of paraffin-embedded rat liver using CEBPA antibody (13-318) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat brain using CEBPA antibody (13-318) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat kidney using CEBPA antibody (13-318) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of mouse lung, using CEBPA antibody (13-318) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat liver using CEBPA antibody (13-318) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using CEBPA antibody (13-318) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using CEBPA antibody (13-318) at dilution of 1:100 (40x lens).

Documents

Further Information

Additional Names:
CEBPA, CEBP, C/EBP-alpha
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain and recognizes the CCAAT motif in the promoters of target genes. The encoded protein functions in homodimers and also heterodimers with CCAAT/enhancer-binding proteins beta and gamma. Activity of this protein can modulate the expression of genes involved in cell cycle regulation as well as in body weight homeostasis. Mutation of this gene is associated with acute myeloid leukemia. The use of alternative in-frame non-AUG (GUG) and AUG start codons results in protein isoforms with different lengths. Differential translation initiation is mediated by an out-of-frame, upstream open reading frame which is located between the GUG and the first AUG start codons.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-205 of human CEBPA (NP_004355.2).
NCBI Gene ID #:
1050
NCBI Official Name:
CCAAT/enhancer binding protein (C/EBP), alpha
NCBI Official Symbol:
CEBPA
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 41kDa
Purification:
Affinity purification
Research Area:
Cancer, Signal Transduction, Transcription
Swissprot #:
P49715
User NOte:
Optimal dilutions for each application to be determined by the researcher.