SLC1A2 Antibody

ProSci
Product Code: PSI-13-320
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-13-320-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using SLC1A2 antibody (13-320) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.
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Immunohistochemistry of paraffin-embedded rat pancreas using SLC1A2 antibody (13-320) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human liver using SLC1A2 antibody (13-320) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse pancreas using SLC1A2 antibody (13-320) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using SLC1A2 antibody (13-320) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.
Immunohistochemistry of paraffin-embedded rat pancreas using SLC1A2 antibody (13-320) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver using SLC1A2 antibody (13-320) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse pancreas using SLC1A2 antibody (13-320) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
EAAT2, EIEE41, GLT-1, HBGT, excitatory amino acid transporter 2, excitotoxic amino acid transporter 2, glutamate/aspartate transporter II, sodium-dependent glutamate/aspartate transporter 2, solute carrier family 1 (glial high affinity glutamate transporter), member 2
Application Note:
WB: 1:500 - 1:5000

IHC: 1:100 - 1:200
Background:
This gene encodes a member of a family of solute transporter proteins. The membrane-bound protein is the principal transporter that clears the excitatory neurotransmitter glutamate from the extracellular space at synapses in the central nervous system. Glutamate clearance is necessary for proper synaptic activation and to prevent neuronal damage from excessive activation of glutamate receptors. Improper regulation of this gene is thought to be associated with several neurological disorders. Alternatively spliced transcript variants of this gene have been identified.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 495-574 of human SLC1A2 (NP_004162.2).
NCBI Gene ID #:
6506
NCBI Official Name:
solute carrier family 1 member 2
NCBI Official Symbol:
SLC1A2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 65kDa
Purification:
Affinity purification
Research Area:
Neuroscience, Signal Transduction
Swissprot #:
P43004
User NOte:
Optimal dilutions for each application to be determined by the researcher.