NTF4 Antibody

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ProSci
Product Code: PSI-13-321
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-13-321-50uL50uL£433.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of HT-1080 cells, using NTF4 antibody (13-321) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 5s.
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Immunohistochemistry of paraffin-embedded human colon using NTF4 antibody (13-321) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human prostate using NTF4 antibody (13-321) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of HT-1080 cells, using NTF4 antibody (13-321) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 5s.
Immunohistochemistry of paraffin-embedded human colon using NTF4 antibody (13-321) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate using NTF4 antibody (13-321) at dilution of 1:100 (40x lens).

Documents

Further Information

Additional Names:
NTF5, neurotrophin 5 (neurotrophin 4/5), NT-4/5, NT4, NT5, NTF4, neurotrophic factor 4, neurotrophic factor 5, neurotrophin 4, neurotrophin 5, NTF5
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
This gene is a member of a family of neurotrophic factors, neurotrophins, that control survival and differentiation of mammalian neurons. The expression of this gene is ubiquitous and less influenced by environmental signals. While knock-outs of other neurotrophins including nerve growth factor, brain-derived neurotrophic factor, and neurotrophin 3 prove lethal during early postnatal development, NTF5-deficient mice only show minor cellular deficits and develop normally to adulthood.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 25-210 of human NTF4 (NP_006170.1).
NCBI Gene ID #:
4909
NCBI Official Name:
neurotrophin 4
NCBI Official Symbol:
NTF4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 35kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Growth Factors, Neuroscience
Swissprot #:
P34130
User NOte:
Optimal dilutions for each application to be determined by the researcher.