ACADS Antibody

ProSci
Product Code: PSI-13-328
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-13-328-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using ACADS antibody (13-328) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.
2 / 4
Immunohistochemistry of paraffin-embedded human liver cancer using ACADS antibody (13-328) at dilution of 1:100 (40x lens).
3 / 4
Immunohistochemistry of paraffin-embedded human liver damage using ACADS antibody (13-328) at dilution of 1:100 (40x lens).
4 / 4
Immunofluorescence analysis of U2OS cells using ACADS antibody (13-328). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using ACADS antibody (13-328) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.
Immunohistochemistry of paraffin-embedded human liver cancer using ACADS antibody (13-328) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver damage using ACADS antibody (13-328) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using ACADS antibody (13-328). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
ACADS, ACAD3, SCAD
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:200
Background:
This gene encodes a tetrameric mitochondrial flavoprotein, which is a member of the acyl-CoA dehydrogenase family. This enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Mutations in this gene have been associated with short-chain acyl-CoA dehydrogenase (SCAD) deficiency. Alternative splicing results in two variants which encode different isoforms.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human ACADS (NP_000008.1).
NCBI Gene ID #:
35
NCBI Official Name:
acyl-CoA dehydrogenase, C-2 to C-3 short chain
NCBI Official Symbol:
ACADS
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 44kDa
Purification:
Affinity purification
Research Area:
Cancer, Signal Transduction
Swissprot #:
P16219
User NOte:
Optimal dilutions for each application to be determined by the researcher.