Hsp60 Antibody

ProSci
Product Code: PSI-13-342
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-13-342-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 4
Western blot analysis of extracts of various cell lines, using Hsp60 antibody (13-342) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
2 / 4
Immunohistochemistry of paraffin-embedded rat kidney using Hsp60 antibody (13-342) at dilution of 1:100 (40x lens).
3 / 4
Immunofluorescence analysis of HeLa cells using Hsp60 antibody (13-342). Blue: DAPI for nuclear staining.
4 / 4
Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug Hsp60 antibody (13-342). Western blot was performed from the immunoprecipitate using Hsp60 antibody (13-342) at a dilition of 1:1000.

Western blot analysis of extracts of various cell lines, using Hsp60 antibody (13-342) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunohistochemistry of paraffin-embedded rat kidney using Hsp60 antibody (13-342) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using Hsp60 antibody (13-342). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug Hsp60 antibody (13-342). Western blot was performed from the immunoprecipitate using Hsp60 antibody (13-342) at a dilition of 1:1000.

Further Information

Additional Names:
60 kDa heat shock protein, mitochondrial, 60 kDa chaperonin, Chaperonin 60, CPN60, Heat shock protein 60, HSP-60, Hsp60, HuCHA60, Mitochondrial matrix protein P1, P60 lymphocyte protein, HSPD1, HSP60
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:100

IP: 1:50 - 1:200
Background:
This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 27-240 of human Hsp60 (NP_002147.2).
NCBI Gene ID #:
3329
NCBI Official Name:
heat shock 60kDa protein 1 (chaperonin)
NCBI Official Symbol:
HSPD1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 61kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cell Cycle, Signal Transduction
Swissprot #:
P10809
User NOte:
Optimal dilutions for each application to be determined by the researcher.