TGM2 Antibody

ProSci
Product Code: PSI-13-350
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-13-350-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using TGM2 antibody (13-350) at 1:300 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
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Immunohistochemistry of paraffin-embedded mouse brain using TGM2 antibody (13-350) (40x lens).
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Immunohistochemistry of paraffin-embedded human lung cancer using TGM2 antibody (13-350) (40x lens).
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Immunohistochemistry of paraffin-embedded human esophagus using TGM2 antibody (13-350) (40x lens).

Western blot analysis of extracts of various cell lines, using TGM2 antibody (13-350) at 1:300 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded mouse brain using TGM2 antibody (13-350) (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using TGM2 antibody (13-350) (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using TGM2 antibody (13-350) (40x lens).

Further Information

Additional Names:
TGM2, TG2, TGC, GNAH, HEL-S-45, G-ALPHA-h
Application Note:
WB: 1:500 - 1:2000

IHC: 1:100 - 1:200
Background:
Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. Finally, the encoded protein is the autoantigen implicated in celiac disease. Two transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant protein of human TGM2
NCBI Gene ID #:
7052
NCBI Official Name:
transglutaminase 2
NCBI Official Symbol:
TGM2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 90kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cancer, Cell Cycle, Immunology, Neuroscience, Signal Transduction
Swissprot #:
P21980
User NOte:
Optimal dilutions for each application to be determined by the researcher.